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Biology · PYQ NEET UG 2021

हिन्दी

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

  1. A
    Ligation
  2. B
    Annealing
  3. C
    Extension
  4. D
    Denaturation
View Answer & Solution

Correct answer · Option D

Denaturation

Explanation

The initial step of PCR is denaturation, which requires a high temperature (around \( 94-98^\circ\text{C} \)) to separate the DNA double strands. If very high temperature is not maintained, denaturation is affected first.
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