Biology · PYQ NEET UG 2021
हिन्दीDuring the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
View Answer & Solution
Correct answer · Option D
Denaturation
Explanation
The initial step of PCR is denaturation, which requires a high temperature (around \( 94-98^\circ\text{C} \)) to separate the DNA double strands. If very high temperature is not maintained, denaturation is affected first.