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Biology · PYQ NEET UG 2022

हिन्दी

Which one of the following statement is not true regarding gel electrophoresis technique?

  1. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
  2. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
  3. The process of extraction of separated DNA strands from gel is called elution.
  4. The separated DNA fragments are stained by using ethidium bromide.

Correct answer · Option 1

The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

Explanation

Ethidium bromide and UV light are used to visualize DNA bands (bright orange), and elution is the extraction process. Chromogenic substrates are used in insertional inactivation with blue-white colonies, not for direct gel electrophoresis staining of DNA bands.

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