Biology · CELL THE UNIT OF LIFE

Which of the following statements regarding the nuclear envelope, perinuclear space, and nucleolus in an interphase eukaryotic cell is fully accurate?

1. The perinuclear space measures \( 1\text{--}5\,\text{nm} \) and completely seals the nucleoplasm, preventing any bidirectional movement of macromolecules.
2. The nucleolus is segregated from the rest of the nucleoplasm by a continuous unit membrane to prevent ribosome assembly during interphase.
3. The perinuclear space (\( 10\text{--}50\,\text{nm} \)) forms a barrier between nuclear materials and cytoplasm, while the nucleolus is a non-membrane-bound structure that is the site of active ribosomal RNA synthesis.
4. Nuclear pores are formed by spontaneous rupture of the outer nuclear membrane alone, leaving the inner membrane intact.