Biology · Principles and Processes in Biotechnology
हिन्दीGiven below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Selection of recombinants using chromogenic substrate (insertional inactivation of beta-galactosidase) is preferred over antibiotic resistance replica plating.\n Reason (R): Antibiotic selection requires simultaneous or consecutive plating on two different media plates, making it cumbersome. \nIn the light of the above statements, choose the correct answer from the options given below:
Correct answer · Option 1
Both (A) and (R) are true and (R) is the correct explanation of (A).
Explanation
Plating on two plates containing different antibiotics is cumbersome; chromogenic substrate differentiates recombinants (white) from non-recombinants (blue) on a single plate.