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Biology · PYQ NEET UG 2021
हिन्दीNowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
Correct answer · Option 1
mutated gene does not appear on a photographic film as the probe has no complementarity with it.
Explanation
In molecular diagnosis, a single-stranded DNA or RNA tagged with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells. Due to mutation, the probe does not have complementarity with the mutated gene, hence it does not bind, and the mutated gene does not appear on the photographic film.
