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Biology · Principles and Processes in Biotechnology

हिन्दी

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Recognition sites for restriction enzymes inside a cloning vector should preferably be single. Reason (R): Presence of more than one recognition site within the vector will generate several fragments, complicating gene cloning. In the light of the above statements, choose the correct answer from the options given below:

  1. Both (A) and (R) are true and (R) is the correct explanation of (A).
  2. Both (A) and (R) are true but (R) is NOT the correct explanation of (A).
  3. is true but (R) is false.
  4. is false but (R) is true.

Correct answer · Option 1

Both (A) and (R) are true and (R) is the correct explanation of (A).

Explanation

Multiple recognition sites would fragment the vector upon digestion, making foreign DNA insertion impossible.
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