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Biology · BIOMOLECULES
हिन्दीWhen the concentration of substrate is progressively increased in an in vitro enzymatic assay, the reaction velocity initially rises and then reaches a plateau (\( V_{\max} \)). Why cannot the velocity be exceeded by further adding more substrate?
Correct answer · Option 1
Because the enzyme molecules are fewer than substrate molecules and all enzyme active sites become fully saturated.
Explanation
The velocity reaches \( V_{\max} \) and does not increase further because the enzyme molecules are limited and their active sites become completely saturated with substrate molecules.
