Biology · Principles and Processes in Biotechnology

A foreign DNA fragment is ligated into the BamHI site of plasmid pBR322. The transformants are selected on a medium containing ampicillin, and subsequently replica-plated onto a medium containing tetracycline. What will be the fate of the recombinant colonies?

1. They will grow on both ampicillin and tetracycline containing media.
2. They will grow on ampicillin-containing medium but not on tetracycline-containing medium.
3. They will grow on tetracycline-containing medium but not on ampicillin-containing medium.
4. They will fail to grow on both ampicillin and tetracycline containing media.