Biology · Principles and Processes in Biotechnology
हिन्दीGiven below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): During PCR, primers are oriented such that the DNA polymerase extends them towards each other across the target sequence.\n Reason (R): DNA polymerases can synthesize new strands only in the 5' to 3' direction. \nIn the light of the above statements, choose the correct answer from the options given below:
Correct answer · Option 1
Both (A) and (R) are true and (R) is the correct explanation of (A).
Explanation
Because DNA polymerases add dNTPs exclusively to the free 3'-OH group (5' to 3' direction), primers anneal to opposite strands at opposite ends directed towards each other.