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Biology · Principles and Processes in Biotechnology

हिन्दी

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): In the heat shock protocol, bacterial cells incubated with recombinant DNA on ice are briefly transferred to 42°C and then immediately returned to ice.\n Reason (R): Heat shock expands cell membrane pores temporarily, enabling efficient uptake of the plasmid DNA into the bacterium. \nIn the light of the above statements, choose the correct answer from the options given below:

  1. Both (A) and (R) are true and (R) is the correct explanation of (A).
  2. Both (A) and (R) are true but (R) is NOT the correct explanation of (A).
  3. is true but (R) is false.
  4. is false but (R) is true.

Correct answer · Option 1

Both (A) and (R) are true and (R) is the correct explanation of (A).

Explanation

Thermal shock from 0°C to 42°C and back transiently alters membrane fluidity and pore dynamics, forcing DNA into the bacterium.
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