Biology · PYQ NEET UG 2023
हिन्दीMain steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.\nA. Insertion of recombinant DNA into the host cell.\nB. Cutting of DNA at specific location by restriction enzyme.\nC. Isolation of desired DNA fragment.\nD. Amplification of gene of interest using PCR.\nChoose the correct answer from the options given below :
Correct answer · Option 2
C, B, D, A
Explanation
The correct sequence of rDNA technology steps starts with isolation of desired DNA (C), cutting with restriction enzymes (B), amplification via PCR (D), and finally insertion into host cells (A). Thus, sequence is C, B, D, A.