Biology · Principles and Processes in Biotechnology

In cloning vector \( \text{pBR322} \), ligation of foreign DNA at the \( \text{BamH I} \) site located within the tetracycline resistance gene results in which of the following outcomes?

Loss of ampicillin resistance due to insertional inactivation
Loss of tetracycline resistance while ampicillin resistance remains intact
Inactivation of both ampicillin and tetracycline resistance genes
Retention of resistance against all available antibiotics

Correct answer · Option 2

Loss of tetracycline resistance while ampicillin resistance remains intact

Explanation

Inserting foreign DNA into the \( \text{BamH I} \) site disrupts the tetracycline resistance gene, leading to loss of tetracycline resistance while ampicillin resistance remains functional.

In cloning vector \( \text{pBR322} \), ligation of foreign DNA at the \( \text{BamH I} \) site located within the tetracycline resistance gene results in which of the following outcomes?

Correct answer · Option 2

Explanation

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In cloning vector \( \text{pBR322} \), ligation of foreign DNA at the \( \text{BamH I} \) site located within the tetracycline resistance gene results in which of the following outcomes?

Correct answer · Option 2

Explanation

Related questions

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