Biology · Principles and Processes in Biotechnology
हिन्दीGiven below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Vector DNA and foreign DNA must be cut with the same restriction enzyme before ligation.\n Reason (R): Cutting with the same restriction enzyme yields DNA fragments possessing complementary sticky ends that can be joined by DNA ligase. \nIn the light of the above statements, choose the correct answer from the options given below:
Correct answer · Option 1
Both (A) and (R) are true and (R) is the correct explanation of (A).
Explanation
Using the same restriction enzyme ensures both host/vector and foreign DNA have identical complementary sticky ends, allowing ligation.