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Biology · Principles and Processes in Biotechnology

हिन्दी

In preparing competent bacterial cells for transformation, treatment with divalent calcium cations directly aids in:

  1. Degrading the peptidoglycan cell wall completely.
  2. Increasing the efficiency with which foreign DNA enters through cell wall pores.
  3. Neutralizing negative charges on vector restriction sites.
  4. Stimulating the host DNA polymerase for plasmid replication.

Correct answer · Option 2

Increasing the efficiency with which foreign DNA enters through cell wall pores.

Explanation

Divalent cations like calcium increase the permeability and efficiency with which hydrophilic DNA enters the bacterium through pores in its cell wall.
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