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Biology · PYQ NEET UG 2025

हिन्दी

In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

  1. Using ampicillin & tetracyclin containing medium plate.
  2. Blue color colonies will be selected.
  3. White color colonies will be selected.
  4. Blue color colonies grown on ampicillin plates can be selected.

Correct answer · Option 3

White color colonies will be selected.

Explanation

Insertion of alien DNA at EcoRI site disrupts the gene for \( \beta \)-galactosidase (insertional inactivation), resulting in non-functional enzyme, so recombinant colonies appear white on selection.
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