Biology · PYQ NEET UG 2025
हिन्दीIn the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Correct answer · Option 3
White color colonies will be selected.
Explanation
Insertion of alien DNA at EcoRI site disrupts the gene for \( \beta \)-galactosidase (insertional inactivation), resulting in non-functional enzyme, so recombinant colonies appear white on selection.