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Biology · Principles and Processes in Biotechnology

हिन्दी

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): EcoRI cuts the DNA sequence \( 5' - \text{GAATTC} - 3' \) between bases G and A, producing sticky ends.\n Reason (R): EcoRI cuts the DNA strands a little away from the centre of the palindrome sites between the same two bases on opposite strands. \nIn the light of the above statements, choose the correct answer from the options given below:

  1. Both (A) and (R) are true and (R) is the correct explanation of (A).
  2. Both (A) and (R) are true but (R) is NOT the correct explanation of (A).
  3. is true but (R) is false.
  4. is false but (R) is true.

Correct answer · Option 1

Both (A) and (R) are true and (R) is the correct explanation of (A).

Explanation

EcoRI produces sticky ends because it cuts away from the center of the palindrome between the G and A bases on both strands.
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