Biology · Principles and Processes in Biotechnology
हिन्दीGiven below are two statements:\n Statement I: Each cycle of Polymerase Chain Reaction involves three steps: denaturation, primer annealing, and extension.\n Statement II: Thermostable DNA polymerase (Taq polymerase) requires RNA primers and catalyzes polymerization in the 3' to 5' direction. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
Correct answer · Option 3
Statement I is correct but Statement II is incorrect.
Explanation
Statement I is correct. Statement II is incorrect because PCR uses synthetic DNA oligonucleotide primers and Taq polymerase polymerizes strictly in the 5' to 3' direction.