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Biology · Principles and Processes in Biotechnology

हिन्दी

Given below are two statements:\n Statement I: Each cycle of Polymerase Chain Reaction involves three steps: denaturation, primer annealing, and extension.\n Statement II: Thermostable DNA polymerase (Taq polymerase) requires RNA primers and catalyzes polymerization in the 3' to 5' direction. \nIn the light of the above statements, choose the most appropriate answer from the options given below:

  1. Both Statement I and Statement II are correct.
  2. Both Statement I and Statement II are incorrect.
  3. Statement I is correct but Statement II is incorrect.
  4. Statement I is incorrect but Statement II is correct.

Correct answer · Option 3

Statement I is correct but Statement II is incorrect.

Explanation

Statement I is correct. Statement II is incorrect because PCR uses synthetic DNA oligonucleotide primers and Taq polymerase polymerizes strictly in the 5' to 3' direction.
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