Biology · PYQ NEET UG 2021

Plasmid pBR322 has Pst I restriction enzyme site within gene \( amp^R \) that confers ampicillin resistance. If this enzyme is used for inserting a gene for \( \beta \)-galactoside production and the recombinant plasmid is inserted in an E.coli strain

1. A
   it will be able to produce a novel protein with dual ability.
2. B
   it will not be able to confer ampicillin resistance to the host cell.
3. C
   the transformed cells will have the ability to resist ampicillin as well as produce \( \beta \)-galactoside.
4. D
   it will lead to lysis of host cell.