Biology · BIOMOLECULES

What is 'activation energy' in the context of an enzymatic reaction?

1. A
   The difference in energy between the initial substrate (S) and the final product (P).
2. B
   The difference between the average energy content of the substrate (S) and that of its transition state.
3. C
   The net kinetic energy possessed by the free enzyme before substrate binding.
4. D
   The thermal energy released when product molecules dissociate from the active site.