Biology · Principles and Processes in Biotechnology

What is the biological significance of insertional inactivation of the \( \beta \)-galactosidase gene in screening recombinant colonies?

1. A
   It ensures that the plasmid lacks any autonomous origin of replication
2. B
   Colonies with recombinant DNA produce blue colour with chromogenic substrate
3. C
   Non-recombinants produce blue coloured colonies while recombinants are colourless
4. D
   It increases the copy number of the vector manifold