OnlineCBT
Biology · Principles and Processes in Biotechnology
Practice questions & answers
Find a question, read its answer and explanation, then practise related questions.
Biology Principles and Processes in Biotechnology · Clear filters
109 questions
Biology · Principles and Processes in Biotechnology
Which of the following vectors is naturally disarmed and modified to deliver genes of interest into a variety of dicotyledonous plants?
Read answer →Biology · Principles and Processes in Biotechnology
Match List-I with List-II: List-I List-II A Elution I Process of taking up recombinant DNA into host B Transformation II Extraction of DNA bands from agarose gel C Palindromic sequence III Sequence reading same forward and backward D Cloning IV Making multiple identical copies of DNA Choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Plasmids and bacteriophages are widely used as cloning vectors in recombinant DNA technology. Reason (R): Plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): DNA fragments stained with ethidium bromide can be directly visualised in normal daylight.\n Reason (R): Ethidium bromide emits bright orange light under visible daylight without needing ultraviolet radiation. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
The European Federation of Biotechnology (EFB) defines biotechnology by integrating both traditional views and modern molecular biotechnology. Which of the following correctly represents the official definition given by EFB?
Read answer →Biology · Principles and Processes in Biotechnology
Taq polymerase used in PCR reactions remains active during high temperature because it is isolated from:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Plasmids are extrachromosomal, autonomously replicating circular DNA molecules found in bacteria. Reason (R): Plasmids possess an origin of replication (\( \text{ori} \)) which enables them to multiply independently of chromosomal DNA control in host cells. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Hind II always cuts DNA molecules at a specific point by recognizing a specific sequence of six base pairs.\n Reason (R): Restriction endonucleases bind to DNA and cut both strands at specific points in their sugar-phosphate backbones. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Which of the following methods involves introducing foreign DNA directly into the nucleus of an animal cell using micro-needles?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): A cloning vector must preferably have only a single recognition site for a given restriction endonuclease.\n Reason (R): Presence of more than one recognition site within the vector generates several fragments, which complicates the gene cloning process. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
In the naming convention of restriction endonuclease enzymes such as EcoRI, what does the letter 'R' represent?
Read answer →Biology · Principles and Processes in Biotechnology
Who constructed the first artificial recombinant DNA molecule by linking an antibiotic resistance gene with a native plasmid from \( \text{Salmonella typhimurium} \) in 1972?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Selection of recombinants using chromogenic substrate (insertional inactivation of beta-galactosidase) is preferred over antibiotic resistance replica plating.\n Reason (R): Antibiotic selection requires simultaneous or consecutive plating on two different media plates, making it cumbersome. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Restriction enzymes belong to a larger class of enzymes called nucleases. The specific function of exonucleases is to:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Biolistics or gene gun method is suitable for introducing foreign DNA into plant cells. Reason (R): Plant cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
During the isolation of genetic material, purified DNA precipitates out after the addition of which of the following substances?
Read answer →Biology · Principles and Processes in Biotechnology
Match List-I with List-II: List-I List-II A EcoRI I Six base pair recognition sequence B Hind II II Derived from Escherichia coli RY 13 C Ethidium bromide III Staining agent used in gel electrophoresis D Agarose IV Natural polymer extracted from sea weeds Choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Recognition sites for restriction enzymes inside a cloning vector should preferably be single. Reason (R): Presence of more than one recognition site within the vector will generate several fragments, complicating gene cloning. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Why does asexual reproduction preserve genetic information while sexual reproduction permits variation?
Read answer →Biology · Principles and Processes in Biotechnology
Which of the following definitions of biotechnology encompasses both traditional fermentation processes and modern molecular genetic engineering as given by the European Federation of Biotechnology (EFB)?
Read answer →Biology · Principles and Processes in Biotechnology
Bioprocess engineering differs from genetic engineering primarily in its focus and methodology. Which of the following best describes the key objective of bioprocess engineering?
Read answer →Biology · Principles and Processes in Biotechnology
A foreign DNA fragment is ligated into the BamHI site of plasmid pBR322. The transformants are selected on a medium containing ampicillin, and subsequently replica-plated onto a medium containing tetracycline. What will be the fate of the recombinant colonies?
Read answer →Biology · Principles and Processes in Biotechnology
