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Biology · Principles and Processes in Biotechnology
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Biology · Principles and Processes in Biotechnology
Match List-I with List-II: List-I List-II A Origin of replication I Enzyme that joins foreign DNA to plasmid B Bioprocess engineering II Sequence responsible for initiating replication C DNA Ligase III Maintenance of sterile ambiance for large-scale growth D Plasmid IV Extrachromosomal autonomously replicating DNA Choose the correct answer from the options given below:
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The first restriction endonuclease isolated and characterized was Hind II. It inspects DNA and cuts it at a specific recognition sequence consisting of how many base pairs?
Read answer →Biology · Principles and Processes in Biotechnology
A continuous culture system is preferred for large-scale production of a recombinant protein because:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements:\n Statement I: Cohen and Boyer isolated an antibiotic resistance gene by cutting out a piece of DNA from a plasmid of Salmonella typhimurium in 1972.\n Statement II: Cutting of DNA at specific locations became possible only with the discovery of DNA ligase enzyme. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
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Given below are two statements: Statement I: \( \text{Hind II} \) is the first restriction endonuclease whose exact nucleotide recognition sequence of six base pairs was characterised. Statement II: Today, over 900 restriction enzymes have been isolated from more than 230 strains of bacteria. In the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
What type of chemical bonds are formed between the sticky ends of DNA fragments when joined by the enzyme DNA ligase?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): In the heat shock protocol, bacterial cells incubated with recombinant DNA on ice are briefly transferred to 42°C and then immediately returned to ice.\n Reason (R): Heat shock expands cell membrane pores temporarily, enabling efficient uptake of the plasmid DNA into the bacterium. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Which of the following enzymes is used to digest plant cell walls to isolate pure genomic DNA?
Read answer →Biology · Principles and Processes in Biotechnology
In 1972, Stanley Cohen and Herbert Boyer constructed the first artificial recombinant DNA molecule by isolating an antibiotic resistance gene from a native plasmid of which organism?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Bioprocess engineering involves maintaining sterile ambience to enable growth of only desired microbes. Reason (R): Unwanted microbial contamination in large-scale cultures can ruin biotechnological product yields. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
In agarose gel electrophoresis, DNA fragments migrate towards the anode because:
Read answer →Biology · Principles and Processes in Biotechnology
During the isolation of genetic material from fungal cells, the cell wall is lysed using which specific enzyme?
Read answer →Biology · Principles and Processes in Biotechnology
In the diagrammatic representation of recombinant DNA technology shown, what is the correct sequence of steps involved after cutting foreign DNA and vector DNA with the same restriction enzyme?
Read answer →Biology · Principles and Processes in Biotechnology
According to the European Federation of Biotechnology (EFB), biotechnology encompasses both traditional view and modern molecular biotechnology. Which of the following components forms the core definition given by EFB?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Sparged stirred-tank bioreactor allows better oxygen mixing compared to simple stirred-tank bioreactor. Reason (R): Sterile air bubbles sparged from the bottom dramatically increase the oxygen transfer area. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
During the visualisation of DNA fragments separated by agarose gel electrophoresis, pure DNA can be observed only after staining with:
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In a continuous culture system, what is the primary operational advantage that maintains cells in their physiologically most active log/exponential phase?
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Given below are two statements: Statement I: Agarose is a natural polymer extracted from sea weeds used as a matrix in gel electrophoresis. Statement II: The smaller the DNA fragment size, the slower it moves through the agarose gel matrix under an electric field. In the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
What are the two core techniques that enabled the birth of modern biotechnology?
Read answer →Biology · Principles and Processes in Biotechnology
Among the two core techniques that enabled the birth of modern biotechnology, 'Genetic engineering' primarily involves:
Read answer →Biology · Principles and Processes in Biotechnology
In cloning vector \( \text{pBR322} \), ligation of foreign DNA at the \( \text{BamH I} \) site located within the tetracycline resistance gene results in which of the following outcomes?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Smaller DNA fragments migrate faster and farther than larger fragments in an agarose gel. Reason (R): The sieving effect of the agarose gel allows smaller fragments to maneuver through the pores more easily. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
