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Biology · Principles and Processes in Biotechnology
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Biology · Principles and Processes in Biotechnology
In agarose gel electrophoresis, DNA fragments move towards the anode because DNA molecules carry a net charge that is:
Read answer →Biology · Principles and Processes in Biotechnology
Why do bacteriophages serve as excellent cloning vectors compared to some plasmids in terms of copy number per bacterial cell?
Read answer →Biology · Principles and Processes in Biotechnology
In the naming convention of restriction enzymes like \( \text{EcoRI} \), what does the letter 'R' denote?
Read answer →Biology · Principles and Processes in Biotechnology
In the construction of the first recombinant DNA by Stanley Cohen and Herbert Boyer in 1972, the antibiotic resistance gene was isolated from which organism and linked to its native plasmid?
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Given below are two statements: Statement I: The convention for naming restriction enzymes dictates that the first letter comes from the genus and the next two letters from the species of the prokaryotic cell. Statement II: Roman numbers following the names of restriction enzymes indicate the order in which the enzymes were isolated from that strain of bacteria. In the light of the above statements, choose the most appropriate answer from the options given below:
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Match List-I with List-II: \n List-I List-II A Denaturation I Addition of primers to single-stranded DNA B Annealing II Thermal melting of dsDNA at high temperature C Extension III Synthesis of new DNA strand using Taq polymerase D Spooling IV Collection of precipitated DNA threads \n Choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Downstream processing in biotechnology involves which of the following sequential stages prior to product marketing?
Read answer →Biology · Principles and Processes in Biotechnology
What is the critical role of the 'origin of replication' (\( \text{ori} \)) sequence when an alien piece of DNA is introduced into a host organism?
Read answer →Biology · Principles and Processes in Biotechnology
What causes restriction enzymes to produce overhanging single-stranded stretches called 'sticky ends' on DNA strands?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Vector DNA and foreign DNA must be cut with the same restriction enzyme before ligation.\n Reason (R): Cutting with the same restriction enzyme yields DNA fragments possessing complementary sticky ends that can be joined by DNA ligase. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Restriction endonucleases inspect the length of a DNA sequence to find their specific recognition sites. Reason (R): Restriction endonucleases cleave both strands of DNA at random positions without sequence specificity. In the light of the above statements, choose the correct answer from the options given below:
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Given below are two statements:\n Statement I: Traditional hybridisation procedures used in plant and animal breeding very often lead to inclusion and multiplication of undesirable genes along with desirable genes.\n Statement II: Genetic engineering techniques like gene cloning and gene transfer overcome this limitation by allowing isolation and introduction of only desirable genes. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Match List-I with List-II: \n List-I List-II A Lysozyme I Fungal cell wall digestion B Cellulase II Bacterial cell wall digestion C Chitinase III Plant cell wall digestion D Ribonuclease IV RNA removal \n Choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
In preparing competent bacterial cells for transformation, treatment with divalent calcium cations directly aids in:
Read answer →Biology · Principles and Processes in Biotechnology
What is the primary function of a foam braker in a simple stirred-tank bioreactor?
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Ethidium bromide is used to stain DNA fragments in agarose gel electrophoresis. Reason (R): Stained DNA fragments can be visualized directly under normal room light without any UV exposure. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece in a process known as elution. Reason (R): Elution allows the purified DNA fragments to be subsequently used in constructing recombinant DNA. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
What is the biological significance of insertional inactivation of the \( \beta \)-galactosidase gene in screening recombinant colonies?
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Techniques like in vitro fertilisation, synthesising a gene, and DNA vaccine development fall under the scope of modern biotechnology.\n Reason (R): The definition given by EFB encompasses both traditional view and modern molecular biotechnology. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Exonucleases and Endonucleases are two classes of nucleases that differ in their mode of action. Which of the following accurately describes the function of exonucleases?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: Statement I: Exonucleases make internal cuts within a DNA strand at specific palindromic sequences. Statement II: Restriction endonucleases add methyl groups to host DNA to protect it from degradation. In the light of the above statements, choose the most appropriate answer from the options given below:
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The ultimate precipitation of purified DNA as fine threads during genetic material isolation is achieved by adding:
Read answer →Biology · Principles and Processes in Biotechnology
