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Biology · Principles and Processes in Biotechnology
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Biology · Principles and Processes in Biotechnology
Given below are two statements:\n Statement I: The origin of replication ('ori') is a DNA sequence that controls the copy number of the linked alien DNA.\n Statement II: Normal E. coli cells naturally possess genes encoding resistance against ampicillin and chloramphenicol. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): DNA cannot easily pass across the lipid bilayer membrane of a bacterial cell without special chemical or physical treatment.\n Reason (R): DNA is a hydrophobic polymer with non-polar nitrogenous bases on its exterior. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Match List-I with List-II: \n List-I List-II A Agrobacterium tumefaciens I Thermostable DNA polymerase source B Thermus aquaticus II Pathogen used for transferring T-DNA into plants C \( \beta \)-galactosidase III Enzyme involved in insertional inactivation D Bioreactor IV Vessel to process large volumes of culture \n Choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Which of the following characteristics is most essential for a cloning vector to facilitate high copy number of the linked DNA in host cells?
Read answer →Biology · Principles and Processes in Biotechnology
Which specific DNA sequence is responsible for initiating replication when an alien piece of DNA is linked to it in a host organism?
Read answer →Biology · Principles and Processes in Biotechnology
Why are single-stranded overhanging stretches created by restriction endonucleases called 'sticky ends'?
Read answer →Biology · Principles and Processes in Biotechnology
Which direct gene transfer technique involves bombarding target plant cells with high-velocity micro-particles coated with foreign DNA?
Read answer →Biology · Principles and Processes in Biotechnology
In a sparged stirred-tank bioreactor, bubbling sterile air through the culture vessel primarily serves to:
Read answer →Biology · Principles and Processes in Biotechnology
In recombinant DNA technology, the selection of recombinants based on insertional inactivation of the beta-galactosidase coding gene produces white colonies because:
Read answer →Biology · Principles and Processes in Biotechnology
Refer to the diagram showing the action of the restriction enzyme EcoRI on vector DNA and foreign DNA. Which specific sequence is cut between G and A bases by EcoRI?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements:\n Statement I: Elution is the process of cutting out separated bands of DNA from agarose gel and extracting them from the gel piece.\n Statement II: Bacteriophages have very high copy numbers per cell because of their high number within bacterial cells. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Refer to the agarose gel electrophoresis diagram (Figure 9.3). Which lane represents the undigested DNA sample, and which end contains the smallest DNA fragments?
Read answer →Biology · Principles and Processes in Biotechnology
Which restriction endonuclease was the first to be discovered and isolated whose functioning depended on a specific nucleotide sequence of six base pairs?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: Statement I: Three basic steps in genetically modifying an organism include identification of DNA with desirable genes, introduction of the identified DNA into the host, and maintenance of introduced DNA in the host and transfer to its progeny. Statement II: Recombinant DNA technology can be accomplished without restriction enzymes if DNA ligase alone is used on foreign DNA. In the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): Stirred-tank bioreactors usually have a curved base.\n Reason (R): A curved base facilitates uniform mixing of the reactor contents and prevents dead zones. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
What term is used for the process of cutting out separated bands of DNA from the agarose gel and extracting them from the gel piece?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): EcoRI cuts the DNA sequence \( 5' - \text{GAATTC} - 3' \) between bases G and A, producing sticky ends.\n Reason (R): EcoRI cuts the DNA strands a little away from the centre of the palindrome sites between the same two bases on opposite strands. \nIn the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Restriction endonucleases inspect the length of a DNA sequence and cut each of the two strands of double helix at specific points. Reason (R): Restriction endonucleases can recognize palindromic nucleotide sequences in the DNA and make cuts in the sugar-phosphate backbones. In the light of the above statements, choose the correct answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
In plasmid vector pBR322, the gene 'rop' codes for:
Read answer →Biology · Principles and Processes in Biotechnology
Which of the following enzymes remains active during the high temperature induced denaturation step of Polymerase Chain Reaction (PCR)?
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements:\n Statement I: If any protein encoding gene is expressed in a heterologous host, it is called a recombinant protein.\n Statement II: Bioreactors are vessels that can process large volumes (100–1000 litres) of biological culture under optimal temperature, pH, and oxygen conditions. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
Given below are two statements:\n Statement I: Each cycle of Polymerase Chain Reaction involves three steps: denaturation, primer annealing, and extension.\n Statement II: Thermostable DNA polymerase (Taq polymerase) requires RNA primers and catalyzes polymerization in the 3' to 5' direction. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
Read answer →Biology · Principles and Processes in Biotechnology
