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Biology · Principles and Processes in Biotechnology
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Biology · Principles and Processes in Biotechnology
Given below are two statements:\n Statement I: Today over 900 restriction enzymes have been isolated from over 230 strains of bacteria.\n Statement II: Roman numbers following the names of restriction enzymes indicate the order in which the enzymes were isolated from that strain of bacteria. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).\n Assertion (A): An alien piece of DNA linked to origin of replication (ori) sequence can replicate and multiply within a host cell.\n Reason (R): Origin of replication is a specific DNA sequence responsible for initiating DNA replication. \nIn the light of the above statements, choose the correct answer from the options given below:
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What is the role of divalent cations like \( \text{Ca}^{2+} \) during the preparation of competent host cells for bacterial transformation?
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Which of the following represents a true palindromic nucleotide sequence recognized by \( \text{EcoRI} \) in double-stranded DNA when read in the \( 5' \rightarrow 3' \) direction?
Read answer →Biology · Principles and Processes in Biotechnology
During the construction of the first recombinant DNA, the cut gene encoding antibiotic resistance was linked with the plasmid vector. Which enzyme catalyzed this covalent joining of cut DNA molecules?
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Ligases join foreign DNA to plasmid vectors by forming phosphodiester bonds between cut ends. Reason (R): DNA ligase acts on cut DNA molecules and joins their ends to reform a continuous sugar-phosphate backbone. In the light of the above statements, choose the correct answer from the options given below:
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Downstream processing is an essential step prior to the marketing of a finished recombinant product. Reason (R): Biosynthetic stage requires continuous extraction of desired proteins without purification steps. In the light of the above statements, choose the correct answer from the options given below:
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Given below are two statements:\n Statement I: In micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell.\n Statement II: In gene gun method, micro-particles of gold or tungsten coated with DNA are bombarded into target plant cells at high velocity. \nIn the light of the above statements, choose the most appropriate answer from the options given below:
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The separation of DNA fragments on agarose gel relies on a sieving effect. What is the source matrix of agarose commonly used in gel electrophoresis?
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Match List-I with List-II: \n List-I List-II A Micro-injection I Gold particles coated with DNA bombarded B Biolistics II Recombinant DNA injected directly into nucleus C Disarmed pathogen III Vectors used to infect and transfer DNA into host D Heat shock IV Incubation of cells on ice followed by 42°C \n Choose the correct answer from the options given below:
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In the Polymerase Chain Reaction (PCR), if 30 successive cycles are completed starting from a single duplex template, approximately how many DNA molecules are generated?
Read answer →Biology · Principles and Processes in Biotechnology
